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DECODR™

Biocomputing made easy and powerful

Accurate. Intuitive. Visual. CRISPR analysis at its full potential.

DECODR™ (Deconvolution of Complex DNA Repair) provides a convenient and user-friendly method to quantify the edits present in CRISPR-edited Sanger sequencing data.

DECODR™ is capable of analyzing a wide range of edits, including:
Indels (both single and multi-guide)
Multi-guide fragment excisions
Compound indels (combined insertion and deletion events)
HDR insertions and single-nucleotide substitutions

Using a computationally efficient algorithm, DECODR™ has virtually no limit on indel size (can quantify deletions several hundred bp long) and can determine the identity of inserted bases. DECODR™ supports batch analysis, in which several sequencing files can be analyzed at a time.

To use DECODR™, all you need to do is perform standard Sanger sequencing reactions on your experimental files. The resulting .ab1 files can be uploaded to DECODR™, and after providing either a text or .ab1 file for the reference sequence, simply submit your files for analysis to receive near NGS-level clarity in your output. For more information on DECODR™, see our paper in The CRISPR Journal.

DECODR™ is available free of charge for all non-profit and academic use on the LatchBio platform. It is also available for licensing by for-profit institutions.

Need more information?

To find out more, email us at geneeditinginstitute@christianacare.org or call
302-623-5306.